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In vivo effects of dentin bonding agents on dentinal fluid movement and intradental nerve activity

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Abstract


The effect of application of dentin bonding agent to the exposed dentin on the intradental nerve activity (INA), dentinal fluid movement and sealing of the dentinal tubules, was investigated in this study. The INA was recorded from the single
pulp
nerve
unit dissected from the inferior alveolar nerve. And specimen of dentin was observed by SEM. Dentinal fluid movement through exposed dentin surface was measured before and after the application of dentin bonding agent.
1. Eight A¥ä-fiber units(conduction velocity : 8.0¡¾4.0m/sec) were identified. 4M NaCl evoked an irregular burst of action potentialswhich ceased immediately after washing.
2. In 4 A¥ä-fiber units, appliction of All Bond 2 completely abolished the INA induced by 4M NaCl. Also, application of Scotchbond Multipurpose(SBMP) totally abolished the INA induced by 4M NaCl in 4 A¥ä-fiber units.
3. Before the application of dentin bonding agent, outward dentinal fluid movement of 10.2¡¾5.7pl ?s-1 ?mm-2 was obsered.. But after the application of dentin boning agent the movement of dentinal fluid was stopped.
4. The gap width of 2-3§­ was formed between exposed dentin and adhesive resin in the specimens applied with dentin bonding agents of All bone 2 and SBMP. But the formation of hybrid layer and the penetration of resin into were dentinal tubules
were
not clearly observed in interface between dentin and adhesive resin.

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